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Heart on A Chip: Label‐Free Isolation and Single Cell Biophysical Phenotyping Analysis of Primary Cardiomyocytes Using Inertial Microfluidics (Small 8/2021)
Author(s) -
Tavassoli Hossein,
Rorimpandey Prunella,
Kang Young Chan,
Carnell Michael,
Brownlee Chris,
Pimanda John E,
Chan Peggy P.Y.,
Chandrakanthan Vashe
Publication year - 2021
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.202170034
Subject(s) - biochip , microfluidics , nanotechnology , regenerative medicine , microfluidic chip , isolation (microbiology) , lab on a chip , computational biology , materials science , biology , microbiology and biotechnology , bioinformatics , stem cell
In article number 2006176, Peggy P. Y. Chan, Vashe Chandrakanthan, and co‐workers develop a label‐free method to purify beating cardiomyocytes from neonatal mouse hearts using a simple particle size‐based inertial microfluidics biochip. Biophysical phenotyping analysis of these sorted cardiomyocytes show distinct and consistent physico‐mechanical and cellular phenotypes of primary cardiomyocytes. This isolation and phenotyping method will serve as a valuable tool to progress our understanding towards regenerative medicine, drug development and diagnostic applications.