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L ‐Arg‐Rich Amphiphilic Dendritic Peptide as a Versatile NO Donor for NO/Photodynamic Synergistic Treatment of Bacterial Infections and Promoting Wound Healing
Author(s) -
Zhu Jingwu,
Tian Jiang,
Yang Chao,
Chen Junpeng,
Wu Lihuang,
Fan Mengni,
Cai Xiaojun
Publication year - 2021
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.202101495
Subject(s) - photodynamic therapy , biofilm , wound healing , amphiphile , singlet oxygen , peptide , chemistry , bacteria , antibiotics , combinatorial chemistry , biochemistry , oxygen , biology , immunology , organic chemistry , genetics , copolymer , polymer
The development of alternative strategies for the efficient treatment of subcutaneous abscesses that do not require the massive use of antibiotics and surgical intervention is urgently needed. Herein, a novel synergistic antibacterial strategy based on photodynamic (PDT) and NO gas therapy is reported, in which, a PDT‐driven NO controllable generation system (Ce6@Arg‐ADP) is developed with l ‐Arg‐rich amphiphilic dendritic peptide (Arg‐ADP) as a carrier. This carrier not only displays superior bacterial association and biofilm penetration performance, but also acts as a versatile NO donor. Following efficient penetration into the interior of the biofilms, Ce6@Arg‐ADP can rapidly produce massive NO via utilizing the H 2 O 2 generated during PDT to oxidize Arg‐ADP to NO and l ‐citrulline, without affecting singlet oxygen ( 1 O 2 ) production. The combination of 1 O 2 and the reactive by‐products of NO offers notable synergistic antibacterial and biofilm eradication effects. Importantly, following efficient elimination of all bacteria from the abscess site, Arg‐ADP can further generate trace quantities of NO to facilitate the angiogenesis and epithelialization of the wound tissues, thereby notably promotes wound healing. Together, this study clearly suggests that Arg‐ADP is a versatile NO donor, and the combination of PDT and NO represents a promising strategy for the efficient treatment of subcutaneous abscesses.