Premium
Precisely Encoded Barcodes through the Structure‐Fluorescence Combinational Strategy: A Flexible, Robust, and Versatile Multiplexed Biodetection Platform with Ultrahigh Encoding Capacities
Author(s) -
Wang Yao,
Chen Cang,
He Jing,
Cao Yimei,
Fang Xiaoxia,
Chi Xiaomei,
Yi Jingwei,
Wu Jiancong,
Guo Qingsheng,
Masoomi Hajar,
Wu Chongzhao,
Ye Jian,
Gu Hongchen,
Xu Hong
Publication year - 2021
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.202100315
Subject(s) - multiplexing , nanotechnology , materials science , fluorescence , encoding (memory) , quantum dot , microbead (research) , microfluidics , barcode , computer science , chemistry , optics , physics , telecommunications , biochemistry , artificial intelligence , operating system
With the rapid development of suspension array technology, microbeads‐based barcodes as the core element with sufficient encoding capacity are urgently required for high‐throughput multiplexed detection. Here, a novel structure‐fluorescence combinational encoding strategy is proposed for the first time to establish a barcode library with ultrahigh encoding capacities. Based on the never revealed transformability of the structural parameters (e.g., porosity and matrix component) of mesoporous microbeads into scattering signals in flow cytometry, the enlargement of codes number has been successfully realized in combination with two other fluorescent elements of fluorescein isothiocyanate isomer I (FITC) and quantum dots (QDs). The barcodes are constructed with precise architectures including FITC encapsulated within mesopores and magnetic nanoparticles as well as QDs immobilized on the outer surface to achieve the ultrahigh encoding level of 300 accompanied with superparamagnetism. To the best of knowledge, it is the highest record of single excitation laser‐based encoding capacity up to now. Moreover, a ten‐plexed tumor markers bioassay based on the tailored‐designed barcodes has been evaluated to confirm their feasibility and effectiveness, and the results indicate that the barcodes platform is a promising and robust tool for practical multiplexed biodetection.