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Protein Thermodynamic Stability: On‐Chip Acousto Thermal Shift Assay for Rapid and Sensitive Assessment of Protein Thermodynamic Stability (Small 41/2020)
Author(s) -
Ding Yonghui,
Ball Kerri A.,
Webb Kristofor J.,
Gao Yu,
D'Alessandro Angelo,
Old William M.,
Stowell Michael H. B.,
Ding Xiaoyun
Publication year - 2020
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.202070224
Subject(s) - microfluidics , thermal stability , protein stability , chip , materials science , chemical stability , thermal , chemistry , chromatography , nanotechnology , thermodynamics , computer science , biochemistry , physics , organic chemistry , telecommunications
In article number 2003506, Xiaoyun Ding and co‐workers present an acousto thermal shift assay (ATSA) utilizing acoustic mechanisms to denature proteins and concentrate protein precipitates for rapid and label‐free assessment of protein thermodynamic changes in real time. The whole process, including sample handling, protein unfolding, and measurement, takes place within a microfluidic chip in less than two minutes, and is faster than other current thermal shift assays.