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Appreciating the First Line of the Human Innate Immune Defense: A Strategy to Model and Alleviate the Neutrophil Elastase‐Mediated Attack toward Bioactivated Biomaterials
Author(s) -
Blum Carina,
Taskin Mehmet Berat,
Shan Junwen,
Schilling Tatjana,
Schlegelmilch Katrin,
Teßmar Jörg,
Groll Jürgen
Publication year - 2021
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.202007551
Subject(s) - innate immune system , biointerface , neutrophil elastase , biomaterial , immune system , elastase , nanotechnology , inflammation , materials science , microbiology and biotechnology , chemistry , immunology , biology , biochemistry , enzyme
Biointerface engineering is a wide‐spread strategy to improve the healing process and subsequent tissue integration of biomaterials. Especially the integration of specific peptides is one promising strategy to promote the regenerative capacity of implants and 3D scaffolds. In vivo, these tailored interfaces are, however, first confronted with the innate immune response. Neutrophils are cells with pronounced proteolytic potential and the first recruited immune cells at the implant site; nonetheless, they have so far been underappreciated in the design of biomaterial interfaces. Herein, an in vitro approach is introduced to model and analyze the neutrophil interaction with bioactivated materials at the example of nano‐bioinspired electrospun surfaces that reveals the vulnerability of a given biointerface design to the contact with neutrophils. A sacrificial, transient hydrogel coating that demonstrates optimal protection for peptide‐modified surfaces and thus alleviates the immediate cleavage by neutrophil elastase is further introduced.