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Nanobiosensor Reports on CDK1 Kinase Activity in Tumor Xenografts in Mice
Author(s) -
Tîlmaciu Carmen Mihaela,
Dinesh Bhimareddy,
Pellerano Morgan,
Diot Sebastien,
Guidetti Mélanie,
Vollaire Julien,
Bianco Alberto,
MénardMoyon Cécilia,
Josserand Véronique,
Morris May C.
Publication year - 2021
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.202007177
Subject(s) - cyclin dependent kinase 1 , kinase , intracellular , microbiology and biotechnology , biosensor , biophysics , chemistry , cell cycle , biology , cell , biochemistry
Abstract Probing the dynamics and quantifying the activities of intracellular protein kinases that coordinate cell growth and division and constitute biomarkers and pharmacological targets in hyperproliferative and pathological disorders remain a challenging task. Here engineering and characterization of a nanobiosensor of the mitotic kinase CDK1, through multifunctionalization of carbon nanotubes with a CDK1‐specific fluorescent peptide reporter, are described. This original reporter of CDK1 activity combines the sensitivity of a fluorescent biosensor with the unique physico‐chemical and biological properties of nanotubes for multifunctionalization and efficient intracellular penetration. The functional versatility of this nanobiosensor enables implementation to quantify CDK1 activity in a sensitive and dose‐dependent fashion in complex biological environments in vitro, to monitor endogenous kinase in living cells and directly within tumor xenografts in mice by fluorescence imaging, thanks to a ratiometric quantification strategy accounting for response relative to concentration in space and in time.