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Metabolic Labeling Mediated Targeting and Thermal Killing of Gram‐Positive Bacteria by Self‐Reporting Janus Magnetic Nanoparticles
Author(s) -
Hou Shuai,
Mahadevegowda Surendra H.,
Lu Derong,
Zhang Kaixi,
ChanPark Mary B.,
Duan Hongwei
Publication year - 2021
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.202006357
Subject(s) - bacteria , magnetic nanoparticles , nanoparticle , chemistry , gram negative bacteria , biochemistry , biophysics , materials science , nanotechnology , biology , escherichia coli , genetics , gene
Nanoparticles have been widely used in detection and killing of bacteria; however, targeting bacteria is still challenging. Delicate design of nanoparticles is required for simultaneous targeting, detection, and therapeutic functions. Here the use of Au/MnFe 2 O 4 (Au/MFO) Janus nanoparticles to target Gram‐positive bacteria via metabolic labeling is reported and realize integrated self‐reporting and thermal killing of bacteria. In these nanoparticles, the Au component is functionalized with tetrazine to target trans ‐cyclooctene group anchored on bacterial cell wall by metabolic incorporation of d ‐amino acids, and the MFO part exhibits peroxidase activity, enabling self‐reporting of bacteria before treatment. The spatial separation of targeting and reporting functions avoids the deterioration of catalytic activity after surface modification. Also important is that MFO facilitates magnetic separation and magnetic heating, leading to easy enrichment and magnetic thermal therapy of labeled bacteria. This method demonstrates that metabolic labeling with d ‐amino acids is a promising strategy to specifically target and kill Gram‐positive bacteria.