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Optical Imaging and High‐Accuracy Quantification of Intracellular Iron Contents
Author(s) -
Ye Dewen,
Li Mingxi,
Xie Yuanyuan,
Chen Bo,
Han Yuexia,
Liu Sijin,
Wei QiHuo,
Gu Ning
Publication year - 2021
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.202005474
Subject(s) - iron oxide , iron oxide nanoparticles , nanoparticle , intracellular , yield (engineering) , microscopy , chemistry , nanotechnology , materials science , optics , physics , biochemistry , organic chemistry , metallurgy
Precise quantification of intracellular iron contents is important to biomedical applications of magnetic nanoparticles. Current approaches for iron quantification rely on specialized instruments while most only yield iron quantities averaged over plenty of cells. Here, a simple and robust approach, combining digital optical microscopy with the Beer–Lambert's law, that allows for imaging stainable iron distribution in individual cells and the quantification of stainable iron contents with an unprecedented accuracy of femtogram per pixel, is presented. It is further shown that this approach enables studying of the internalization and reduction dynamics of super‐paramagnetic iron oxide nanoparticles (SPIONs) by stem cells in single cell level.