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High‐Fidelity Determination and Tracing of Small Extracellular Vesicle Cargoes
Author(s) -
Chen Xiaohui,
Jia Mei,
Liu Lianhua,
Qiu Xiaopei,
Zhang Hong,
Yu Xingle,
Gu Wei,
Qing Guangchao,
Li Qingmei,
Hu Xiaolin,
Wang Ruixuan,
Zhao Xianxian,
Zhang Liangliang,
Wang Xianfeng,
Durkan Colm,
Wang Nan,
Wang Guixue,
Luo Yang
Publication year - 2020
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.202002800
Subject(s) - tracing , biophysics , nanotechnology , extracellular vesicles , fluorescence , molecular beacon , molecular motor , computational biology , biology , chemistry , dna , microbiology and biotechnology , materials science , computer science , biochemistry , physics , oligonucleotide , quantum mechanics , operating system
Direct tracing of small extracellular vesicle (sEV) cargoes holds unprecedented importance for elucidating the mechanisms involved in intercellular communication. However, high‐fidelity determination of sEVs’ molecular cargoes in situ has yet to be achieved due to the difficulty in transporting molecular probes into intact sEVs. Herein, a fLuorescent Intracellular‐Guided Hairpin‐Tetrahedron (fLIGHT) nanoprobe is described for direct visualization of sEV microRNAs in situ. Integrating the advantages of nondestructive sEV penetration via DNA origami and single‐nucleotide discrimination as well as wash‐free fluorescence readout using a hairpin probe, the proposed approach enables high‐fidelity fluorescence visualization of sEVs’ microRNA without RNA extraction or leakage, demonstrating the potential of on‐site tracing of sEV cargoes. This strategy opens an avenue to establishing universal molecular detection and labeling platforms that can facilitate both sEV‐derived fundamental biological studies and molecular diagnostics.