Premium
Anti‐Atherogenic Effect of Stem Cell Nanovesicles Targeting Disturbed Flow Sites
Author(s) -
Yoon JeongKee,
Kim DaeHyun,
Kang MiLan,
Jang HyeonKi,
Park HyunJi,
Lee Jung Bok,
Yi Se Won,
Kim HyeSeon,
Baek Sewoom,
Park Dan Bi,
You Jin,
Lee SeongDeok,
Sei Yoshitaka,
Ahn Song Ih,
Shin Young Min,
Kim Chang Soo,
Bae Sangsu,
Kim YongTae,
Sung HakJoon
Publication year - 2020
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.202000012
Subject(s) - mesenchymal stem cell , ligation , stem cell , cell , endothelial stem cell , microbiology and biotechnology , monocyte , cancer research , cell growth , inflammation , medicine , biology , immunology , in vitro , biochemistry
Abstract Atherosclerosis development leads to irreversible cascades, highlighting the unmet need for improved methods of early diagnosis and prevention. Disturbed flow formation is one of the earliest atherogenic events, resulting in increased endothelial permeability and subsequent monocyte recruitment. Here, a mesenchymal stem cell (MSC)‐derived nanovesicle (NV) that can target disturbed flow sites with the peptide GSPREYTSYMPH (PREY) (PMSC‐NVs) is presented which is selected through phage display screening of a hundred million peptides. The PMSC‐NVs are effectively produced from human MSCs (hMSCs) using plasmid DNA designed to functionalize the cell membrane with PREY. The potent anti‐inflammatory and pro‐endothelial recovery effects are confirmed, similar to those of hMSCs, employing mouse and porcine partial carotid artery ligation models as well as a microfluidic disturbed flow model with human carotid artery‐derived endothelial cells. This nanoscale platform is expected to contribute to the development of new theragnostic strategies for preventing the progression of atherosclerosis.