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Single Cell Genomics: Whole Genome Sequencing of Single Circulating Tumor Cells Isolated by Applying a Pulsed Laser to Cell‐Capturing Microstructures (Small 37/2019)
Author(s) -
Kim Okju,
Lee Daewon,
Chungwon Lee Amos,
Lee Yongju,
Bae Hyung Jong,
Lee HanByoel,
Kim Ryong Nam,
Han Wonshik,
Kwon Sunghoon
Publication year - 2019
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201970196
Subject(s) - circulating tumor cell , genomics , pulsed laser , microfluidics , cell , single cell analysis , genome , fluorescence , dna sequencing , materials science , computational biology , laser , biology , cancer , dna , nanotechnology , gene , genetics , optics , metastasis , physics
Isolating one rare cell in a second: Single circulating tumor cells (CTCs) within their protein expression contexts are analyzed through next generation sequencing for genome‐wide analysis in article number 1902607 by Sunghoon Kwon and co‐workers. Whole blood from a metastatic breast cancer patient is processed through a microfluidic chip. CTCs are selected via fluorescence imaging and the fluorescently stained CTCs are isolated with a near‐infrared pulsed laser device without contact.