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Enzyme Immobilization: Enhanced Enzyme Activity through Scaffolding on Customizable Self‐Assembling Protein Filaments (Small 20/2019)
Author(s) -
Lim Samuel,
Jung Gi Ahn,
Glover Dominic J.,
Clark Douglas S.
Publication year - 2019
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201970104
Subject(s) - scaffold protein , scaffold , enzyme , immobilized enzyme , biophysics , chemistry , nanotechnology , self assembly , materials science , biochemistry , biology , biomedical engineering , engineering , signal transduction
In article number 1805558 , Douglas S. Clark and co‐workers report a versatile scaffolding platform based on a self‐assembling filamentous protein, gamma‐prefoldin (γ‐PFD), that can immobilize enzymes in a customizable fashion. The scaffold attachment can position fluorescent proteins in nanometer proximity or enhance the intrinsic catalytic activity of the immobilized enzymes.

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