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Photobleaching: Improving the Sensitivity of Fluorescence‐Based Immunoassays by Photobleaching the Autofluorescence of Magnetic Beads (Small 3/2019)
Author(s) -
Roth Shira,
Hadass Orr,
Cohen Meir,
Verbarg Jasenka,
Wilsey Jennifer,
Danielli Amos
Publication year - 2019
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201970016
Subject(s) - autofluorescence , photobleaching , fluorescence , detection limit , materials science , fluorescence recovery after photobleaching , fluorescence lifetime imaging microscopy , chemistry , nuclear magnetic resonance , optics , chromatography , physics
In fluorescence‐based assays, the autofluorescence of capture surfaces produces strong background noise. In article number 1803751 , Amos Danielli and co‐workers use photobleaching as a method to reduce the autofluorescence of magnetic beads, a commonly used capture surface. The technique yields a 3‐fold improvement in the limit of detection and signal‐to‐noise ratio of a fluorescence‐based immunoassay.