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Neurochemistry: Rapid Growth Cone Uptake and Dynein‐Mediated Axonal Retrograde Transport of Negatively Charged Nanoparticles in Neurons Is Dependent on Size and Cell Type (Small 2/2019)
Author(s) -
Lesniak Anna,
Kilinc Devrim,
Blasiak Agata,
Galea George,
Simpson Jeremy C.,
Lee Gil U.
Publication year - 2019
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201970012
Subject(s) - calcein , axoplasmic transport , biophysics , growth cone , somatic cell , chemistry , nanoparticle , neuron , dynein , nanotechnology , anatomy , neuroscience , biology , microbiology and biotechnology , microtubule , materials science , axon , biochemistry , membrane , gene
In article number 1803758 , Gil U. Lee and co‐workers present a novel microfluidics device which makes the study of nanoparticle uptake and transport in neuron cultures possible. The somatic chamber at the top of this fluorescence image shows somato‐dendritic nanoparticle (red) uptake in the primary cortical neurons. At the end of a 6 h incubation period, neurons with axons in the axonal chamber at the bottom of the image are labelled with Calcein (green) to distinguish them from neurons with axons in the somatic chamber.