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Cellular Stemness Maintenance of Human Adipose‐Derived Stem Cells on ZnO Nanorod Arrays
Author(s) -
Kong Ying,
Ma Baojin,
Liu Feng,
Chen Duo,
Zhang Shan,
Duan Jiazhi,
Huang Yuan,
Sang Yuanhua,
Wang Jianjun,
Li Dong,
Liu Hong,
Wang Shuhua
Publication year - 2019
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201904099
Subject(s) - klf4 , stem cell , microbiology and biotechnology , nanotopography , cancer stem cell , adipose tissue , regeneration (biology) , adult stem cell , cellular differentiation , materials science , biology , sox2 , embryonic stem cell , biochemistry , gene
Ever‐growing tissue regeneration and other stem cell therapies cause pressing need for large population of self‐renewable stem cells. However, stem cells gradually lose their stemness after long‐term in vitro cultivation. In this study, a ZnO nanorod (ZnO NR) array is used to maintain the stemness of human adipose‐derived stem cells (hADSCs). The results prove that after culturing hADSCs on ZnO NRs for 3 weeks, the stemness genes and protein expression level are higher than that on culture plates and ZnO film. ZnO NRs can maintain stemness of hADSCs without inhibiting the cell proliferation and oriented differentiation capabilities. KLF4 (Kruppel‐like factor 4) is a Zn 2+ ‐binding gene that plays a vital role in cell proliferation and differentiation. Sustained Zn 2+ release and the increased expression of KLF4 can be detected, suggesting that ZnO NRs have efficiently released Zn 2+ for stemness maintenance. Taken together, the nanotopography of ZnO NRs and the Zn 2+ release synergistically facilitate stemness maintenance. This study has provided a powerful tool for directing cell fate, maintaining stemness, and realizing the expansion of stem cells in vitro, which will open a new route for the manufacture of large populations of stem cells and fulfilling the growing demand for the cell therapy market.