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Adipose‐Derived Biogenic Nanoparticles for Suppression of Inflammation
Author(s) -
Tian Ming,
Ticer Taylor,
Wang Qikun,
Walker Sierra,
Pham Anthony,
Suh Annie,
Busatto Sara,
Davidovich Irina,
AlKharboosh Rawan,
LewisTuffin Laura,
Ji Baoan,
QuisHinojosa Alfredo,
Talmon Yeshayahu,
Shapiro Shane,
Rückert Felix,
Wolfram Joy
Publication year - 2020
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201904064
Subject(s) - adipose tissue , mesenchymal stem cell , inflammation , chemistry , extracellular , secretion , microvesicles , extracellular vesicle , microbiology and biotechnology , proinflammatory cytokine , nanobiotechnology , nanoparticle , immunology , nanotechnology , materials science , medicine , biology , biochemistry , microrna , gene
Extracellular vesicles secreted from adipose‐derived mesenchymal stem cells (ADSCs) have therapeutic effects in inflammatory diseases. However, production of extracellular vesicles (EVs) from ADSCs is costly, inefficient, and time consuming. The anti‐inflammatory properties of adipose tissue‐derived EVs and other biogenic nanoparticles have not been explored. In this study, biogenic nanoparticles are obtained directly from lipoaspirate, an easily accessible and abundant source of biological material. Compared to ADSC‐EVs, lipoaspirate nanoparticles (Lipo‐NPs) take less time to process (hours compared to months) and cost less to produce (clinical‐grade cell culture facilities are not required). The physicochemical characteristics and anti‐inflammatory properties of Lipo‐NPs are evaluated and compared to those of patient‐matched ADSC‐EVs. Moreover, guanabenz loading in Lipo‐NPs is evaluated for enhanced anti‐inflammatory effects. Apolipoprotein E and glycerolipids are enriched in Lipo‐NPs compared to ADSC‐EVs. Additionally, the uptake of Lipo‐NPs in hepatocytes and macrophages is higher. Lipo‐NPs and ADSC‐EVs have comparable protective and anti‐inflammatory effects. Specifically, Lipo‐NPs reduce toll‐like receptor 4‐induced secretion of inflammatory cytokines in macrophages. Guanabenz‐loaded Lipo‐NPs further suppress inflammatory pathways, suggesting that this combination therapy can have promising applications for inflammatory diseases.