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Self‐Assembled Collagen Microparticles by Aerosol as a Versatile Platform for Injectable Anisotropic Materials
Author(s) -
Lama Milena,
Fernandes Francisco M.,
Marcellan Alba,
Peltzer Juliette,
Trouillas Marina,
Banzet Sébastien,
Grosbot Marion,
Sanchez Clément,
GiraudGuille MarieMadeleine,
Lataillade JeanJacques,
Coulomb Bernard,
Boissière Cédric,
Nassif Nadine
Publication year - 2020
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201902224
Subject(s) - extracellular matrix , mesenchymal stem cell , tissue engineering , nanotechnology , materials science , regeneration (biology) , cell encapsulation , biophysics , chemistry , biomedical engineering , microbiology and biotechnology , biochemistry , medicine , biology
Extracellular matrices (ECM) rich in type I collagen exhibit characteristic anisotropic ultrastructures. Nevertheless, working in vitro with this biomacromolecule remains challenging. When processed, denaturation of the collagen molecule is easily induced in vitro avoiding proper fibril self‐assembly and further hierarchical order. Here, an innovative approach enables the production of highly concentrated injectable collagen microparticles, based on collagen molecules self‐assembly, thanks to the use of spray‐drying process. The versatility of the process is shown by performing encapsulation of secretion products of gingival mesenchymal stem cells (gMSCs), which are chosen as a bioactive therapeutic product for their potential efficiency in stimulating the regeneration of a damaged ECM. The injection of collagen microparticles in a cell culture medium results in a locally organized fibrillar matrix. The efficiency of this approach for making easily handleable collagen microparticles for encapsulation and injection opens perspectives in active tissue regeneration and 3D bioprinted scaffolds.