Premium
ZnS/C/MoS 2 Nanocomposite Derived from Metal–Organic Framework for High‐Performance Photo‐Electrochemical Immunosensing of Carcinoembryonic Antigen
Author(s) -
Wei Qiuxi,
Wang Chen,
Li Ping,
Wu Tsunghsueh,
Yang Nianjun,
Wang Xing,
Wang Yanying,
Li Chunya
Publication year - 2019
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201902086
Subject(s) - materials science , detection limit , nanocomposite , carcinoembryonic antigen , nuclear chemistry , raman spectroscopy , nanoparticle , x ray photoelectron spectroscopy , fourier transform infrared spectroscopy , dielectric spectroscopy , scanning electron microscope , analytical chemistry (journal) , chemical engineering , nanotechnology , electrochemistry , chemistry , chromatography , electrode , medicine , physics , engineering , cancer , composite material , optics
A hexafluorophosphate ionic liquid is used as a functional monomer to prepare a metal–organic framework (Zn‐MOF). Zn‐MOF is used as a template for MoS 2 nanosheets synthesis and further carbonized to yield light‐responsive ZnS/C/MoS 2 nanocomposites. Zn‐MOF, carbonized‐Zn‐MOF, and ZnS/C/MoS 2 nanocomposites are characterized by Fourier transform infrared spectroscopy, transmission electron microscopy, X‐ray diffraction pattern, scanning electron microscopy (SEM), element mapping, Raman spectroscopy, X‐ray photoelectron spectroscopy, fluorescence, and nitrogen‐adsorption analysis. Carcinoembryonic antigen (CEA) is selected as a model to construct an immunosensing platform to evaluate the photo‐electrochemical (PEC) performances of ZnS/C/MoS 2 nanocomposites. A sandwich‐type PEC immunosensor is fabricated by immobilizing CEA antibody (Ab 1 ) onto the ZnS/C/MoS 2 /GCE surface, subsequently binding CEA and the alkaline phosphatase‐gold nanoparticle labeled CEA antibody (ALP‐Au‐Ab 2 ). The catalytic conversion of vitamin C magnesium phosphate produces ascorbic acid (AA). Upon being illuminated, AA can react with photogenerated holes from ZnS/C/MoS 2 nanocomposites to generate a photocurrent for quantitative assay. Under optimized experimental conditions, the PEC immunosensor exhibits excellent analytical characteristics with a linear range from 2.0 pg mL −1 to 10.0 ng mL −1 and a detection limit of 1.30 pg mL −1 (S/N = 3). The outstanding practicability of this PEC immunosensor is demonstrated by accurate assaying of CEA in clinical serum samples.