Premium
Stem Cell Imaging: Effective Labeling of Primary Somatic Stem Cells with BaTiO 3 Nanocrystals for Second Harmonic Generation Imaging (Small 8/2018)
Author(s) -
Sugiyama Nami,
Sonay Ali Y.,
Tussiwand Roxanne,
Cohen Bruce E.,
Pantazis Periklis
Publication year - 2018
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201870036
Subject(s) - stem cell , haematopoiesis , materials science , somatic cell , cell , nanoparticle , nanotechnology , nanocrystal , second harmonic imaging microscopy , live cell imaging , second harmonic generation , biophysics , microbiology and biotechnology , chemistry , biology , biochemistry , optics , laser , physics , gene
In article number 1703386 , Periklis Pantazis and co‐workers use Second Harmonic Generating (SHG) nanoprobes to label, with high contrast, clinically relevant hematopoietic stem cells (HSCs) by establishing an effective cell labeling procedure without affecting the HSC differentiation potential. Quantitative single‐cell analysis reveals that freshly isolated HSCs show limited nanoparticle uptake, whereas HSCs entering a proliferative state display a 2.5‐fold increase in uptake.