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High‐Performance Integrated Enzyme Cascade Bioplatform Based on Protein–BiPt Nanochain@Graphene Oxide Hybrid Guided One‐Pot Self‐Assembly Strategy
Author(s) -
Liu Yan,
Zheng Yuanlin,
Chen Zhen,
Qin Yuling,
Guo Rong
Publication year - 2019
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201804987
Subject(s) - nanotechnology , graphene , artificial enzyme , materials science , cascade , oxide , enzyme , chemistry , biochemistry , chromatography , metallurgy
Nanozymes provide new opportunities for facilitating next generation artificial enzyme cascade platforms. However, the fabrication of high‐performance integrated artificial enzyme cascade (IAEC) bioplatforms based on nanozymes remains a great challenge. A facile and effective self‐assembly strategy for constructing an IAEC system based on an inorganic/protein hybrid nanozyme, β‐casein‐BiPt nanochain@GO (CA‐BiPtNC@GO) nanohybrid with unique physicochemical surface properties and hierarchical structures, is introduced here. Due to the synergetic effect of the protein, GO, and Bi 3+ , the hybrid acts as highly adaptable building blocks to immobilize natural enzymes directly and noncovalently without the loss of enzyme activity. Simultaneously, the CA‐BiPtNC@GO nanohybrid exhibits outstanding peroxidase‐mimicking activity and works well with natural oxidases, resulting in prominent activity in catalyzing cascade reactions. As a result, the proposed IAEC bioplatform exhibits excellent sensitivity with a wide linear range of 0.5 × 10 ‐6 to 100 × 10 ‐6 m and a detection limit of 0.05 × 10 ‐6 m for glucose. Meticulous design of ingenious hierarchically nanostructured nanozymes with unique physicochemical surface properties can provide a facile and efficient way to immobilize and stabilize nature enzymes using self‐assembly instead of chemical processes, and fill the gap in developing robust nanozyme–triggered IAEC systems with applications in the environment, sensing, and synthetic biology.