Premium
Standing Surface Acoustic Wave (SSAW)‐Based Fluorescence‐Activated Cell Sorter
Author(s) -
Ren Liqiang,
Yang Shujie,
Zhang Peiran,
Qu Zhiguo,
Mao Zhangming,
Huang PoHsun,
Chen Yuchao,
Wu Mengxi,
Wang Lin,
Li Peng,
Huang Tony Jun
Publication year - 2018
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201801996
Subject(s) - interdigital transducer , materials science , microfluidics , cell sorting , fluorescence , computer science , nanotechnology , transducer , biomedical engineering , computer hardware , acoustics , cell , chemistry , optics , engineering , biochemistry , physics
Microfluidic fluorescence‐activated cell sorters (μFACS) have attracted considerable interest because of their ability to identify and separate cells in inexpensive and biosafe ways. Here a high‐performance μFACS is presented by integrating a standing surface acoustic wave (SSAW)‐based, 3D cell‐focusing unit, an in‐plane fluorescent detection unit, and an SSAW‐based cell‐deflection unit on a single chip. Without using sheath flow or precise flow rate control, the SSAW‐based cell‐focusing technique can focus cells into a single file at a designated position. The tight focusing of cells enables an in‐plane‐integrated optical detection system to accurately distinguish individual cells of interest. In the acoustic‐based cell‐deflection unit, a focused interdigital transducer design is utilized to deflect cells from the focused stream within a minimized area, resulting in a high‐throughput sorting ability. Each unit is experimentally characterized, respectively, and the integrated SSAW‐based FACS is used to sort mammalian cells (HeLa) at different throughputs. A sorting purity of greater than 90% is achieved at a throughput of 2500 events s −1 . The SSAW‐based FACS is efficient, fast, biosafe, biocompatible and has a small footprint, making it a competitive alternative to more expensive, bulkier traditional FACS.