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Photothermal Control of Heat‐Shock Protein Expression at the Single Cell Level
Author(s) -
Robert Hadrien M. L.,
Savatier Julien,
Vial Stéphanie,
Verghese Jacob,
Wattellier Benoit,
Rigneault Hervé,
Monneret Serge,
Polleux Julien,
Baffou Guillaume
Publication year - 2018
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201801910
Subject(s) - photothermal therapy , shearing (physics) , materials science , laser , interferometry , fusion , shock (circulatory) , fluorescence , colloidal gold , biophysics , nanotechnology , nanoparticle , optoelectronics , optics , composite material , biology , medicine , linguistics , philosophy , physics
Laser heating of individual cells in culture recently led to seminal studies in cell poration, fusion, migration, or nanosurgery, although measuring the local temperature increase in such experiments remains a challenge. Here, the laser‐induced dynamical control of the heat‐shock response is demonstrated at the single cell level, enabled by the use of light‐absorbing gold nanoparticles as nanosources of heat and a temperature mapping technique based on quadriwave lateral shearing interferometry (QLSI) measurements. As it is label‐free, this approach does not suffer from artifacts inherent to previously reported fluorescence‐based temperature‐mapping techniques and enables the use of any standard fluorescent labels to monitor in parallel the cell's response.