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Single Molecule DNA Resensing Using a Two‐Pore Device
Author(s) -
Zhang Yuning,
Liu Xu,
Zhao Yanan,
Yu JenKan,
Reisner Walter,
Dunbar William B.
Publication year - 2018
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201801890
Subject(s) - nanopore , materials science , molecule , nanotechnology , borosilicate glass , microchannel , membrane , ionic bonding , microfluidics , chemistry , ion , composite material , biochemistry , organic chemistry
A nanofluidic device is presented that, enables independent sensing and resensing of a single DNA molecule translocating through two nanopores with sub‐micrometer spacing. The device concept is based upon integrating a thin nitride membrane with microchannels etched in borosilicate glass. Pores, coupled to each microchannel, are connected via a fluid‐filled half‐space on the device backside, enabling translocation of molecules across each pore in sequence. Critically, this approach allows for independent application of control voltage and measurement of trans‐pore ionic current at each of the two pores, leading to 1) controlled assessment of molecular time of flight, 2) voltage‐tuned selective molecule recapture, and 3) ability to acquire two correlated translocation signatures for each molecule analyzed. Finally, the rare cocapture of a single chain threading simultaneously through each of the two pores is reported.