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Light‐Triggered Genome Editing: Cre Recombinase Mediated Gene Editing with Near‐Infrared Light
Author(s) -
Morales Demosthenes P.,
Morgan Erin N.,
McAdams Meghan,
Chron Amanda B.,
Shin Jeong Eun,
Zasadzinski Joseph A.,
Reich Norbert O.
Publication year - 2018
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201800543
Subject(s) - nitrilotriacetic acid , recombinase , genome editing , endosome , fluorescence , chemistry , gene , biophysics , materials science , genome , biology , biochemistry , chelation , physics , quantum mechanics , recombination , intracellular , organic chemistry
A light‐activated genome editing platform based on the release of enzymes from a plasmonic nanoparticle carrier when exposed to biocompatible near‐infrared light pulses is described. The platform relies on the robust affinity of polyhistidine tags to nitrilotriacetic acid in the presence of copper which is attached to double‐stranded nucleic acids self‐assembled on the gold nanoparticle surface. A protein fusion of the Cre recombinase containing a TAT internalization peptide sequence to achieve endosomal localization is also employed. High‐resolution gene knock‐in of a red fluorescent reporter is observed using a commercial two‐photon microscope. High‐throughput irradiation is described to generate useful quantities of edited cells.