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Microgels: Centering Single Cells in Microgels via Delayed Crosslinking Supports Long‐Term 3D Culture by Preventing Cell Escape (Small 22/2017)
Author(s) -
Kamperman Tom,
Henke Sieger,
Visser Claas Willem,
Karperien Marcel,
Leijten Jeroen
Publication year - 2017
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201770120
Subject(s) - microfluidics , materials science , cell , modular design , nanotechnology , enhanced data rates for gsm evolution , cell culture , term (time) , biophysics , chemical engineering , chemistry , computer science , physics , engineering , biology , genetics , operating system , quantum mechanics , telecommunications , biochemistry
In article number 1603711 , Marcel Karperien, Jeroen Leijten, and co‐workers present the first microfluidic approach that enables long‐term 3D single cell culture by centering cells in microgels in a facile, modular, and widely applicable manner. A typical cell microencapsulation event using the delayed crosslinking approach enables a cell to be positioned at the outer edge of a droplet, and by delaying on‐chip crosslinking, the cell is allowed to move towards the center of the droplet. Subsequent crosslinking finally results in an individual cell being encapsulated in the microgel's center.