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Electrochemical Biosensor Composed of Silver Ion‐Mediated dsDNA on Au‐Encapsulated Bi 2 Se 3 Nanoparticles for the Detection of H 2 O 2 Released from Breast Cancer Cells
Author(s) -
Mohammadniaei Mohsen,
Yoon Jinho,
Lee Taek,
Bharate Bapurao G.,
Jo Jinhee,
Lee Donghyun,
Choi JeongWoo
Publication year - 2018
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201703970
Subject(s) - biosensor , materials science , electrochemistry , nanotechnology , selenide , nanoparticle , cyclic voltammetry , detection limit , scanning electrochemical microscopy , analytical chemistry (journal) , electrode , nuclear chemistry , chemistry , selenium , organic chemistry , chromatography , metallurgy
A newly developed electrochemical biosensor composed of a topological insulator (TI) and metallic DNA (mDNA) is fabricated. The bismuth selenide nanoparticle (Bi 2 Se 3 NP) is synthesized and sandwiched between the gold electrode and another Au‐deposited thin layer (Bi 2 Se 3 @Au). Then, eight‐silver‐ion mediated double‐stranded DNA (mDNA) is immobilized onto the substrate (Bi 2 Se 3 @Au‐mDNA) for the further detection of hydrogen peroxide. The Bi 2 Se 3 NP acts as the electrochemical‐signal booster, while unprecedentedly its encapsulation by the Au thin layer keeps the TI surface states protected, improves its electrochemical‐signal stability and provides an excellent platform for the subsequent covalent immobilization of the mDNA through Au–thiol interaction. Electrochemical results show that the fabricated biosensor represents much higher Ag + redox current (≈10 times) than those electrodes prepared without Bi 2 Se 3 @Au. The characterization of the Bi 2 Se 3 @Au‐mDNA film is confirmed by atomic force microscopy, scanning tunneling microscopy, and cyclic voltammetry. The proposed biosensor shows a dynamic range of 00.10 × 10 −6 m to 27.30 × 10 −6 m , very low detection limit (10 × 10 −9 m ), unique current response (1.6 s), sound H 2 O 2 recovery in serum, and substantial capability to classify two breast cancer subtypes (MCF‐7 and MDA‐MB‐231) based on their difference in the H 2 O 2 generation, offering potential applications in the biomedicine and pharmacology fields.