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Chiral Shell Core–Satellite Nanostructures for Ultrasensitive Detection of Mycotoxin
Author(s) -
Cai Jiarong,
Hao Changlong,
Sun Maozhong,
Ma Wei,
Xu Chuanlai,
Kuang Hua
Publication year - 2018
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201703931
Subject(s) - ochratoxin a , circular dichroism , aptamer , nanostructure , biosensor , detection limit , materials science , nanotechnology , mycotoxin , linear range , satellite , chemistry , chromatography , physics , crystallography , food science , biology , genetics , astronomy
Herein, the design of a DNA‐based chiral biosensor is described utilizing the self‐assembly of shell core–gold (Au) satellite nanostructures for the detection of mycotoxin, ochratoxin A (OTA). The assembly of core–satellite nanostructures based on OTA‐aptamer binding exhibits a strong chiral signal with an intense circular dichroism (CD) peak. The integrity of the assembly of core–satellite nanostructures is limited to some extent in the presence of different levels of OTA. Correspondingly, the chiral intensity of assembly is weakened with increasing OTA concentrations, allowing quantitative determination of the target. The developed chiral sensor shows an excellent linear relationship between the CD signal and concentrations of OTA in the range of 0.1–5 pg mL −1 with a limit of detection as low as 0.037 pg mL −1 . The effectiveness of the biosensor in a sample of red wine is verified and a good recovery rate is obtained. These results suggest that the strategy has great potential for practical application.