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Layered Aggregation with Steric Effect: Morphology‐Homogeneous Semiconductor MoS 2 as an Alternative 2D Probe for Visual Immunoassay
Author(s) -
Li Bang Lin,
Peng Li Yu,
Zou Hao Lin,
Li Ling Jie,
Luo Hong Qun,
Li Nian Bing
Publication year - 2018
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201703560
Subject(s) - materials science , nanotechnology , bioanalysis , immunoassay , nanoparticle , naked eye , homogeneous , steric effects , electrophoresis , phase (matter) , molecular binding , chromatography , chemistry , molecule , detection limit , organic chemistry , physics , antibody , immunology , biology , thermodynamics
Abstract Liquid‐phase exfoliation routes unavoidably generate 2D nanostructures with inhomogeneous morphologies. Herein, thickness‐dependent sorting of exfoliated nanostructures is achieved via a treatment of differential‐zone centrifugation in the surfactant aqueous phase. With this approach, homogeneous MoS 2 nanosheets are obtained, and due to the intrinsic semiconducting characteristics, those 2D nanosheets are endowed with desired optical properties, rivaling classic gold nanoparticles in sensing applications. Furthermore, MoS 2 nanosheets with high uniformity and chemical inertness are coupled with proteins, exhibiting high performance in stability and anti‐interferences for bioanalysis. As a consequence of aggregation‐induced steric effect, distinguishing running shifts of antibody‐anchored conjugates in gel electrophoresis are visually responsive to those specific antigens. This assay enables the easy and fast monitoring of tumor biomarkers just according to “naked‐eye” identification of band location in electrophoresis results, which are presented by an alternative visual probe of 2D MoS 2 ‐protein conjugates. The developed visual immunoassay with the synergistic effect of gel electrophoresis techniques and 2D semiconductors pushes significant progress in “home‐made” tests for disease early diagnosis.