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Cu 2+ ‐Modified Metal–Organic Framework Nanoparticles: A Peroxidase‐Mimicking Nanoenzyme
Author(s) -
Chen WeiHai,
VázquezGonzález Margarita,
Kozell Anna,
Cecconello Alessandro,
Willner Itamar
Publication year - 2018
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201703149
Subject(s) - chemistry , gluconic acid , chemiluminescence , catalysis , luminol , photochemistry , nicotinamide adenine dinucleotide , horseradish peroxidase , redox , glucose oxidase , peroxidase , fluorescence , metal ions in aqueous solution , oxidase test , nuclear chemistry , metal , inorganic chemistry , nad+ kinase , organic chemistry , enzyme , physics , quantum mechanics
The synthesis and characterization of UiO‐type metal–organic framework nanoparticles (NMOFs) composed of Zr 4+ ions bridged by 2,2′‐bipyridine‐5,5′‐dicarboxylic acid ligands and the postmodification of the NMOFs with Cu 2+ ions are described. The resulting Cu 2+ ‐modified NMOFs, Cu 2+ ‐NMOFs, exhibit peroxidase‐like catalytic activities reflected by the catalyzed oxidation of Amplex‐Red to the fluorescent Resorufin by H 2 O 2 , the catalyzed oxidation of dopamine to aminochrome by H 2 O 2 , and the catalyzed generation of chemiluminescence in the presence of luminol/H 2 O 2 . Also, the Cu 2+ ‐NMOFs mimic NADH peroxidase functions and catalyze the oxidation of dihydronicotinamide adenine dinucleotide, NADH, to nicotinamide adenine dinucleotide, NAD + , in the presence of H 2 O 2 . The Cu 2+ ‐NMOFs‐catalyzed generation of chemiluminescence in the presence of luminol/H 2 O 2 is used to develop a glucose sensor by monitoring the H 2 O 2 formed by the aerobic oxidation of glucose to gluconic acid in the presence of glucose oxidase. Furthermore, loading the Cu 2+ ‐NMOFs with fluorescein and activating the catalyzed generation of chemiluminescence in the presence of luminol/H 2 O 2 yield an efficient chemiluminescence resonance energy transfer (CRET) process to the fluorescein reflected by the activation of the fluorescence of the dye (λ = 520 nm, CRET efficiency 35%).