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Myoglobin and Polydopamine‐Engineered Raman Nanoprobes for Detecting, Imaging, and Monitoring Reactive Oxygen Species in Biological Samples and Living Cells
Author(s) -
Kumar Sumit,
Kumar Amit,
Kim GyeongHwan,
Rhim WonKyu,
Hartman Kevin L.,
Nam JwaMin
Publication year - 2017
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201701584
Subject(s) - reactive oxygen species , raman scattering , raman spectroscopy , nanotechnology , myoglobin , plasmon , nanoparticle , materials science , biophysics , chemistry , biochemistry , biology , optoelectronics , physics , optics
Highly reliable detection, imaging, and monitoring of reactive oxygen species (ROS) are critical for understanding and studying the biological roles and pathogenesis of ROS. This study describes the design and synthesis of myoglobin and polydopamine‐engineered surface‐enhanced Raman scattering (MP‐SERS) nanoprobes with strong, tunable SERS signals that allow for specifically detecting and imaging ROS sensitively and quantitatively. The study shows that a polydopamine nanolayer can facilitate the modification of Raman‐active myoglobins and satellite Au nanoparticles (s‐AuNPs) to a plasmonic core AuNP (c‐AuNP) in a controllable manner and the generation of plasmonically coupled hot spots between a c‐AuNP and s‐AuNPs that can induce strong SERS signals. The six‐coordinated Fe(III)‐OH 2 of myoglobins in plasmonic hotspots is reacted with ROS (H 2 O 2 , •OH, and O 2 − ) to form Fe(IV)O. The characteristic Raman peaks of Fe(IV)O from the Fe‐porphyrin is used to analyze and quantify ROS. This chemistry allows for these probes to detect ROS in solution and image ROS in cells in a highly designable, specific, and sensitive manner. This work shows that these MP‐SERS probes allow for detecting and imaging ROS to differentiate cancerous cells from noncancerous cells. Importantly, for the first time, SERS‐based monitoring of the autophagy process in living cells under starvation conditions is validated.