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A Graphene Quantum Dots–Hypochlorite Hybrid System for the Quantitative Fluorescent Determination of Total Antioxidant Capacity
Author(s) -
Wang Min,
Chen Jie,
Liu Chenghao,
Qiu Jianding,
Wang Xiaomeng,
Chen Peng,
Xu Chenjie
Publication year - 2017
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201700709
Subject(s) - hypochlorite , graphene , oxidative stress , antioxidant , chemistry , antioxidant capacity , fluorescence , superoxide dismutase , quenching (fluorescence) , quantum dot , biochemistry , nanotechnology , biophysics , materials science , organic chemistry , biology , physics , quantum mechanics
Antioxidants play a major part in the prevention and impairment of oxidative stress‐induced damages and diseases. Evaluating the antioxidants activity/capacity in food and biological fluid is proved to be useful for the diagnosis and treatment of these oxidative stress‐induced diseases. Herein, a graphene quantum dots (GQDs)–hypochlorite system to detect the antioxidants including nonenzymatic and enzymatic antioxidants in the biological fluid is proposed. The detection principle is based on the fact that antioxidants can protect the fluorescence of GQDs from hypochlorite‐caused quenching by acting as the scavengers of hypochlorite. The GQDs–hypochlorite system allows the accurate quantification of the total antioxidant capacity (TAC) of commercial drinks as well as the extracellular superoxide dismutase (SOD) secretion upon stimulation of cytokines or hyperglycemia. This system shows the excellent analytical recoveries for commercial drinks (>89.9%) and good consistency with ELISA testing for SOD secretion in cell‐conditioned medium. These results demonstrate the ability and reliability of the GQD–hypochlorite system for detecting and quantifying TAC in real drinks and complex biological fluids.