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DNA‐SMART: Biopatterned Polymer Film Microchannels for Selective Immobilization of Proteins and Cells
Author(s) -
Schneider AnnKathrin,
Nikolov Pavel M.,
Giselbrecht Stefan,
Niemeyer Christof M.
Publication year - 2017
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201603923
Subject(s) - nanotechnology , polymer , microfluidics , materials science , chemistry , chemical engineering , composite material , engineering
A novel SMART module, dubbed “DNA‐SMART” (DNA substrate modification and replication by thermoforming) is reported, where polymer films are premodified with single‐stranded DNA capture strands, microthermoformed into 3D structures, and postmodified with complementary DNA‐protein conjugates to realize complex biologically active surfaces within microfluidic devices. As a proof of feasibility, it is demonstrated that microchannels presenting three different proteins on their inner curvilinear surface can be used for selective capture of cells under flow conditions.