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Design and Analysis of Localized DNA Hybridization Chain Reactions
Author(s) -
Bui Hieu,
Miao Vincent,
Garg Sudhanshu,
Mokhtar Reem,
Song Tianqi,
Reif John
Publication year - 2017
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201602983
Subject(s) - dna , chain reaction , dna–dna hybridization , nucleic acid thermodynamics , hybridization probe , in situ hybridization , substrate (aquarium) , chemistry , biophysics , materials science , biology , biochemistry , photochemistry , base sequence , gene , ecology , gene expression
Theoretical models of localized DNA hybridization reactions on nanoscale substrates indicate potential benefits over conventional DNA hybridization reactions. Recently, a few approaches have been proposed to speed‐up DNA hybridization reactions; however, experimental confirmation and quantification of the acceleration factor have been lacking. Here, a system to investigate localized DNA hybridization reactions on a nanoscale substrate is presented. The system consists of six metastable DNA hairpins that are tethered to a long DNA track. The localized DNA hybridization reaction of the proposed system is triggered by a DNA strand which initiates the subsequent self‐assembly. Fluorescence kinetics indicates that the half‐time completion of a localized DNA hybridization chain reaction is six times faster than the same reaction in the absence of the substrate. The proposed system provides one of the first known quantification of the speed‐up of DNA hybridization reactions due to the locality effect.