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5 nm Silver Nanoparticles Amplify Clinical Features of Atopic Dermatitis in Mice by Activating Mast Cells
Author(s) -
Kang HanGoo,
Kim Seungjae,
Lee Kwang Hoon,
Jin Shan,
Kim Seo Hyeong,
Lee Kangtaek,
Jeon Hyungjoon,
Song YoungGeon,
Lee SangWon,
Seo Jinwon,
Park Soomin,
Choi InHong
Publication year - 2017
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201602363
Subject(s) - degranulation , allergen , immunoglobulin e , immunology , hydrogen peroxide , mast cell , reactive oxygen species , chemistry , atopic dermatitis , allergy , tryptase , silver nanoparticle , microbiology and biotechnology , materials science , nanoparticle , medicine , biology , biochemistry , nanotechnology , antibody , receptor
The triggering effect of silver nanoparticles (NPs) on the induction of allergic reactions is evaluated, by studying the activation of mast cells and the clinical features of atopic dermatitis in a mouse model. Granule release is induced in RBL‐2H3 mast cells by 5 nm, but not 100 nm silver NPs. Increases in the levels of reactive oxygen species (hydrogen peroxide and mitochondrial superoxide) and intracellular Ca ++ in mast cells are induced by 5 nm silver NPs. In a mouse model of atopic dermatitis induced by a mite allergen, the skin lesions are more severe and appear earlier in mice treated simultaneously with 5 nm silver NPs and allergen compared with mice treated with allergen alone or 100 nm silver NPs and allergen. The histological findings reveal that number of tryptase‐positive mast cells and total IgE levels in the serum increase in mice treated with 5 nm silver NPs and allergen. The results in this study indicate that cotreatment with 5 nm silver NPs stimulates mast cell degranulation and induces earlier and more severe clinical alterations in allergy‐prone individuals.