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Construction of Asymmetrical Hexameric Biomimetic Motors with Continuous Single‐Directional Motion by Sequential Coordination
Author(s) -
Zhao Zhengyi,
Zhang Hui,
Shu Dan,
Montemagno Carlo,
Ding Baoquan,
Li Jingyuan,
Guo Peixuan
Publication year - 2017
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201601600
Subject(s) - random hexamer , molecular motor , dimer , biophysics , motor protein , protein subunit , dna origami , atpase , dna , chemistry , stereochemistry , physics , crystallography , nanotechnology , biology , biochemistry , materials science , microtubule , microbiology and biotechnology , nuclear magnetic resonance , enzyme , gene
The significance of bionanomotors in nanotechnology is analogous to mechanical motors in daily life. Here the principle and approach for designing and constructing biomimetic nanomotors with continuous single‐directional motion are reported. This bionanomotor is composed of a dodecameric protein channel, a six‐pRNA ring, and an ATPase hexamer. Based on recent elucidations of the one‐way revolving mechanisms of the phi29 double‐stranded DNA (dsDNA) motor, various RNA and protein elements are designed and tested by single‐molecule imaging and biochemical assays, with which the motor with active components has been constructed. The motor motion direction is controlled by three operation elements: (1) Asymmetrical ATPase with ATP‐interacting domains for alternative DNA binding/pushing regulated by an arginine finger in a sequential action manner. The arginine finger bridges two adjacent ATPase subunits into a non‐covalent dimer, resulting in an asymmetrical hexameric complex containing one dimer and four monomers. (2) The dsDNA translocation channel as a one‐way valve. (3) The hexameric pRNA ring geared with left‐/right‐handed loops. Assessments of these constructs reveal that one inactive subunit of pRNA/ATPase is sufficient to completely block motor function (defined as K = 1), implying that these components work sequentially based on the principle of binomial distribution and Yang Hui's triangle.