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Chemically Engineered Nanoparticle–Protein Interface for Real‐Time Cellular Oxidative Stress Monitoring
Author(s) -
Jiang Ying,
Wang Ming,
Hardie Joseph,
Tonga Gulen Yesilbag,
Ray Moumita,
Xu Qiaobing,
Rotello Vincent M.
Publication year - 2016
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201600986
Subject(s) - hydrogen peroxide , chemistry , green fluorescent protein , nanoparticle , covalent bond , fluorescence , peroxide , combinatorial chemistry , colloidal gold , nanotechnology , materials science , organic chemistry , biochemistry , physics , quantum mechanics , gene
A co‐engineered nanoparticle/protein peroxide detector is created . This system features a gold nanoparticle functionalized with a galactose headgroup (AuNP‐Gal) that reacts covalently with a boronate‐modified green fluorescent protein (PB‐GFP). Boronate acid‐saccharide complexation between PB‐GFP and AuNP‐Gal affords a highly stable assembly. This complex is disrupted by peroxide, allowing quantitative and selective monitoring of hydrogen peroxide production in real time.