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Single‐Cell Tracking: Label‐Free Tracking of Single Organelle Transportation in Cells with Nanometer Precision Using a Plasmonic Imaging Technique (Small 24/2015)
Author(s) -
Yang Yunze,
Yu Hui,
Shan Xiaonan,
Wang Wei,
Liu Xianwei,
Wang Shaopeng,
Tao gjian
Publication year - 2015
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201570139
Subject(s) - tracking (education) , plasmon , nanometre , nanotechnology , organelle , materials science , single cell analysis , optoelectronics , cell , physics , optics , chemistry , biology , microbiology and biotechnology , psychology , pedagogy , biochemistry
Scattering from plamonic waves leaves a characteristic “V” shape feature of a small moving organelle, which allows N. Tao and co‐workers to accurately track it in 3D. Parallel kymographs of the tracked organelle show its x,y position versus time. On page 2878, this fast, label‐free method is used to study intracellular dynamics with nanometer tracking accuracy and millisecond temporal resolution.