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Microfluidics: Microfluidic Single‐Cell Analysis with Affinity Beads (Small 22/2015)
Author(s) -
Werner Michael,
Palankar Raghavendra,
Arm Loïc,
Hovius Ruud,
Vogel Horst
Publication year - 2015
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201570128
Subject(s) - microfluidics , bead , single cell analysis , cell , flow cytometry , cytometry , biophysics , population , cytosol , chemistry , nanotechnology , materials science , biochemistry , microbiology and biotechnology , biology , enzyme , demography , sociology , composite material
To investigate functional heterogeneity between individuals in a cell population, affinity beads are transferred into living mammalian cells by H. Vogel and co‐workers. On page 2607, beads coated with specific capture agents efficiently bind and up‐concentrate cytosolic target proteins on their surface. For single‐cell analysis, an optically trapped bead with bound target proteins is extracted from its host cell in a microfluidic device. Compared to classical chemical cytometry, this method has the potential to reach high analytical sensitivity and throughput for single‐cell analysis in many biomedical fields.