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Probes: Single‐Cell Detection of mRNA Expression Using Nanofountain‐Probe Electroporated Molecular Beacons (Small 20/2015)
Author(s) -
GiraldoVela Juan P.,
Kang Wonmo,
McNaughton Rebecca L.,
Zhang Xuemei,
Wile Brian M.,
Tsourkas Andrew,
Bao Gang,
Espinosa Horacio D.
Publication year - 2015
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201570112
Subject(s) - electroporation , molecular beacon , cell , microbiology and biotechnology , gene expression , messenger rna , single cell analysis , population , cell culture , biology , gene , genetics , medicine , environmental health , oligonucleotide
H. D. Espinosa and co‐workers demonstrate a method for real‐time monitoring of gene expression in adherent cells. The method consists of delivering mRNA‐detecting molecular beacons into target cells by nanofountain probe electroporation (NFP‐E). On page 2386, the NFP‐E experimental technique enables temporal studies of the same cell without disturbing cell growth, which could be particularly useful for studies of cell differentiation or for probing specific cells within a cell co‐culture population.