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Imaging: Compact Halo‐Ligand‐Conjugated Quantum Dots for Multicolored Single‐Molecule Imaging of Overcrowding GPCR Proteins on Cell Membranes (Small 12/2015)
Author(s) -
Komatsuzaki Akihito,
Ohyanagi Tatsuya,
Tsukasaki Yoshikazu,
Miyanaga Yukihiro,
Ueda Masahiro,
Jin Takashi
Publication year - 2015
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201570067
Subject(s) - quantum dot , materials science , molecule , ligand (biochemistry) , diffraction , fluorescence , conjugated system , crystallography , nanotechnology , chemistry , optics , physics , polymer , receptor , biochemistry , organic chemistry , composite material
In single‐molecule fluorescence imaging, the optical resolution is restricted by the diffraction limit of light wavelength, which is approximately 200 nm when using visible light emission. M. Ueda, T. Jin, and co‐workers show on page 1396 that the discrimination of individual GPCR proteins within the optical diffraction limit can be achieved by using multicolored single‐molecule imaging with compact halo‐ligand conjugated quantum dots. Three‐colored single‐molecule imaging enables increased detection of the GPCR proteins by a factor of 3 compared to one‐colored single‐molecule imaging.