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Magnetic Tweezers: Probing DNA Helicase Kinetics with Temperature‐Controlled Magnetic Tweezers (Small 11/2015)
Author(s) -
Gollnick Benjamin,
Carrasco Carolina,
Zuttion Francesca,
Gilhooly Neville S.,
Dillingham Mark S.,
MorenoHerrero Fernando
Publication year - 2015
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201570059
Subject(s) - magnetic tweezers , optical tweezers , kinetics , dna , superparamagnetism , molecule , tweezers , helicase , biophysics , materials science , nanotechnology , a dna , chemistry , chemical physics , magnetic field , crystallography , physics , optics , magnetization , biology , biochemistry , classical mechanics , rna , gene , organic chemistry , quantum mechanics
The kinetics of double‐stranded DNA processing are captured at single‐molecule resolution by F. Moreno‐Herrero and co‐workers with a thermally stabilized magnetic tweezers microscope. An individual protein complex called “AddAB” links one end of a DNA molecule to a superparamagnetic bead that is subjected to an upwards‐directed external force. On page 1273, upon addition of the biological fuel ATP, AddAB starts to move along and unwind the two DNA strands, dragging the microsphere behind it. The average translocation velocity increases exponentially with temperature and depends on the ATP concentration.