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Cell Surface Receptors: Rapid Quantification of Live Cell Receptors Using Bioluminescence in a Flow‐Based Microfluidic Device (Small 8/2015)
Author(s) -
Ramji Ramesh,
Cheong Cheong Fook,
Hirata Hiroaki,
Rahman Abdur Rub Abdur,
Lim Chwee Teck
Publication year - 2015
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201570050
Subject(s) - microfluidics , bioluminescence , sensitivity (control systems) , receptor , nanotechnology , materials science , cell , biological system , biophysics , chemistry , biology , electronic engineering , engineering , biochemistry
C. T. Lim and co‐workers describe a rapid and sensitive bioluminescence‐based microfluidic method for quantifying receptor numbers on live cells. On page 943, this integrated, lens‐free optical platform allows the determination of signals from the cell surface with high sensitivity. Compared to conventional approaches, the combined use of bioluminescence and microfluidics makes it safe to use, reduces background noise, improves sensitivity, requires smaller sample volumes, and allows high‐throughput sampling over thousands of cells.