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Intracellular Delivery of a Planar DNA Origami Structure by the Transferrin‐Receptor Internalization Pathway
Author(s) -
Schaffert David H.,
Okholm Anders H.,
Sørensen Rasmus S.,
Nielsen Jesper S.,
Tørring Thomas,
Rosen Christian B.,
Kodal Anne Louise B.,
Mortensen Michael R.,
Gothelf Kurt V.,
Kjems Jørgen
Publication year - 2016
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201503934
Subject(s) - dna origami , transferrin , internalization , dna , transferrin receptor , biophysics , nanotechnology , drug delivery , microbiology and biotechnology , ligand (biochemistry) , confocal microscopy , chemistry , materials science , cell , receptor , biochemistry , biology
DNA origami provides rapid access to easily functionalized, nanometer‐sized structures making it an intriguing platform for the development of defined drug delivery and sensor systems. Low cellular uptake of DNA nanostructures is a major obstacle in the development of DNA‐based delivery platforms. Herein, significant strong increase in cellular uptake in an established cancer cell line by modifying a planar DNA origami structure with the iron transport protein transferrin (Tf) is demonstrated. A variable number of Tf molecules are coupled to the origami structure using a DNA‐directed, site‐selective labeling technique to retain ligand functionality. A combination of confocal fluorescence microscopy and quantitative (qPCR) techniques shows up to 22‐fold increased cytoplasmic uptake compared to unmodified structures and with an efficiency that correlates to the number of transferrin molecules on the origami surface.