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Bioorthogonal Click Chemistry‐Based Synthetic Cell Glue
Author(s) -
Koo Heebeom,
Choi Myunghwan,
Kim Eunha,
Hahn Sei Kwang,
Weissleder Ralph,
Yun Seok Hyun
Publication year - 2015
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201502972
Subject(s) - bioorthogonal chemistry , click chemistry , chemistry , cell , in vivo , biophysics , cell adhesion , covalent bond , fluorescence microscope , nanotechnology , jurkat cells , tetrazine , fluorescence , combinatorial chemistry , materials science , biochemistry , t cell , biology , organic chemistry , physics , microbiology and biotechnology , immune system , quantum mechanics , immunology
Artificial methods of cell adhesion can be effective in building functional cell complexes in vitro, but methods for in vivo use are currently lacking. Here, a chemical cell glue based on bioorthogonal click chemistry with high stability and robustness is introduced. Tetrazine (Tz) and trans ‐cyclooctene (TCO) conjugated to the cell surface form covalent bonds between cells within 10 min in aqueous conditions. Glued, homogeneous, or heterogeneous cell pairs remain viable and stably attached in a microfluidic flow channel at a shear stress of 20 dyn cm −2 . Upon intravenous injection of assembled Jurkat T cells into live mice, fluorescence microscopy shows the trafficking of cell pairs in circulation and their infiltration into lung tissues. These results demonstrate the promising potential of chemically glued cell pairs for various applications ranging from delivering therapeutic cells to studying cell–cell interactions in vivo.