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DNA Polymer Brush Patterning through Photocontrollable Surface‐Initiated DNA Hybridization Chain Reaction
Author(s) -
Huang Fujian,
Zhou Xiang,
Yao Dongbao,
Xiao Shiyan,
Liang Haojun
Publication year - 2015
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201501826
Subject(s) - dna , polymer brush , polymer , biochip , materials science , dna–dna hybridization , nanotechnology , biophysics , chemistry , polymerization , biology , biochemistry , composite material
The fabrication of DNA polymer brushes with spatial resolution onto a solid surface is a crucial step for biochip research and related applications, cell‐free gene expression study, and even artificial cell fabrication. Here, for the first time, a DNA polymer brush patterning method is reported based on the photoactivation of an ortho‐nitrobenzyl linker‐embedded DNA hairpin structure and a subsequent surface‐initiated DNA hybridization chain reaction (HCR). Inert DNA hairpins are exposed to ultraviolet light irradiation to generate DNA duplexes with two active sticky ends (toeholds) in a programmable manner. These activated DNA duplexes can initiate DNA HCR to generate multifunctional patterned DNA polymer brushes with complex geometrical shapes. Different multifunctional DNA polymer brush patterns can be fabricated on certain areas of the same solid surface using this method. Moreover, the patterned DNA brush surface can be used to capture target molecules in a desired manner.