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Single‐Layered MoS 2 –PEI–PEG Nanocomposite‐Mediated Gene Delivery Controlled by Photo and Redox Stimuli
Author(s) -
Kim Jinhwan,
Kim Hyunwoo,
Kim Won Jong
Publication year - 2016
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201501655
Subject(s) - polyethylenimine , nanosheet , nanocomposite , materials science , photothermal therapy , gene delivery , nanomaterials , biophysics , nanotechnology , photothermal effect , polymer , peg ratio , endosome , intracellular , chemistry , transfection , gene , biochemistry , biology , finance , economics , composite material
Stimuli‐responsive gene delivery systems maximize therapeutic efficacy by controlling the cytosolic conveyance and rate of effective gene release. We present herein a hybrid nanocomposite composed of a 2D nanomaterial, MoS 2 , modified by attaching two polymers (polyethylenimine (PEI) and polyethylenglycol (PEG)) via disulfide bonds. This MoS 2 –PEI–PEG nanocomposite interacts with DNA by electrostatic interaction, and accordingly forms a nanosized complex with high stability. Photothermal conversion of MoS 2 nanosheet is employed in order to induce photothermally triggered endosomal escape upon the near infrared light irradiation. After endosomal escape, polymers are detached from the MoS 2 nanosheet by the intracellular reducing agent, glutathione (GSH), resulting in effective gene release from the nanocomposite. This sequential process initiated by external and internal stimuli remarkably enhances gene delivery efficiency by effective endosomal escape and gene release without severe cytotoxicity. Our rationally designed MoS 2 nanocomposite provides a spatiotemporally controllable platform to deliver genetic material into cells.