Premium
Transient Absorption: A New Modality for Microscopic Imaging of Nanomaterials in Living Cells
Author(s) -
Chen Tao,
Huang Yanyi
Publication year - 2015
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201500814
Subject(s) - nanomaterials , absorption (acoustics) , ultrafast laser spectroscopy , materials science , transient (computer programming) , microscopy , microscope , nanotechnology , laser , optoelectronics , optics , computer science , physics , composite material , operating system
Transient absorption is a secondary absorption that happens after a material has been excited through primary absorption. Different mechanisms can contribute to transient absorption. This universal photophysical process exists in almost all types of nanomaterials, making it an ideal modality to monitor the location, dynamics, and interactions of nanomaterials in living cells, tissues, or animals. With two beams of lasers and a scanning microscope, transient absorption microscopy is able to acquire high‐resolution, 3D images at high speed, without the need for labeling. Through time‐delay adjustments of pulse trains, this novel method can also reveal background‐free images of specific nanomaterials, even with the interference of high concentrations of fluorophores.