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Development of a High‐Throughput Functional Screen Using Nanowell‐Assisted Cell Patterning
Author(s) -
Ozkumur Ayca Yalcin,
Goods Brittany A.,
Love J. Christopher
Publication year - 2015
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201500674
Subject(s) - scalability , computer science , throughput , computational biology , human immunodeficiency virus (hiv) , nanotechnology , information retrieval , biology , materials science , database , telecommunications , virology , wireless
Living‐cell‐based screens can facilitate lead discovery of functional therapeutics of interest. A versatile and scalable method is reported that uses dense arrays of nanowells for imparting defined patterns on monolayers of cells. It is shown that this approach can coordinate a multi‐component biological assay by designing and implementing a high‐throughput, functional nanoliter‐scale neutralization assay to identify neutralizing antibodies against HIV.