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Biosensors: On‐line SERS Detection of Single Bacterium Using Novel SERS Nanoprobes and A Microfluidic Dielectrophoresis Device (Small 22/2014)
Author(s) -
Lin HsingYing,
Huang ChenHan,
Hsieh WenHsin,
Liu LingHsuan,
Lin YuanChuen,
Chu ChiaChun,
Wang ShiTing,
Kuo ITing,
Chau LaiKwan,
Yang ChiouYing
Publication year - 2014
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201470137
Subject(s) - dielectrophoresis , biosensor , nanotechnology , materials science , microfluidics , multiplex , detection limit , colloidal gold , plasmon , surface enhanced raman spectroscopy , nanoparticle , raman spectroscopy , optoelectronics , chemistry , raman scattering , chromatography , optics , biology , bioinformatics , physics
On‐line multiplex SERS detection of pathogenic bacteria is demonstrated at a single‐cell level. On page 4700, L.‐K. Chau, C.‐Y. Yang, and co‐workers prepare SERS nanoprobes via immobilization of antibodies on silica‐coated, dye‐induced aggregate beads of gold nanoparticles (AuNPs). The beads give an enhanced Raman signal due to well‐defined plasmonic hot spots at junctions between AuNPs. In combination with dielectrophoresis, they improve the sensitivity of SERS‐barcode biosensing strategies beyond that of whole‐cell ELISA. The practical detection limit over 10 min is 70 CFU mL −1 .