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Fluorescence Imaging: Super‐Resolution Biological Microscopy Using Virtual Imaging by a Microsphere Nanoscope (Small 9/2014)
Author(s) -
Yang Hui,
Moullan Norman,
Auwerx Johan,
Gijs Martin A. M.
Publication year - 2014
Publication title -
small
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.785
H-Index - 236
eISSN - 1613-6829
pISSN - 1613-6810
DOI - 10.1002/smll.201470055
Subject(s) - microsphere , nanoscopic scale , resolution (logic) , fluorescence , materials science , microscopy , fluorescence microscope , nanostructure , fluorescence lifetime imaging microscopy , microscope , diffraction , image resolution , nanotechnology , optics , physics , chemical engineering , computer science , artificial intelligence , engineering
Super‐resolution imaging of sub‐cellular organelles and nanostructures is presented by M. A. M. Gijs and co‐workers on page 1712. This is achieved by simply putting highrefractive index glass microspheres on a sample in combination with a microscope immersion objective for observation. The microsphere projects the sample's nearfield nanoscopic features into the far‐field, generating a magnified virtual image and demonstrating a resolution four times better than that corresponding to the classical diffraction limit.